01 Bacteria
Cell Wall
.
- Gm +: thicker peptidoglycan cell wall, take up stain
- Gm -: outer membrane unique
Peptidoglycan
.
Unique Cell Wall
Cell Membrane
ECT and oxidative phosphorylation in bacteria happens here. Contains enzymes and carrier molecules.
Gm positive membrane
Gm negative membrane
- Periplasm also contains peptidoglycan
- Pts very very sick with gram negative bacteria
LPS
- on outer membrane of gm -
- Lipid A makes patients sick
Capsules
- SHiNE SKiS
Quellung reaction
Immune reaction against capsules
- Capsules resistance to phagocytosis
- opsonin marks for phagocytes
- also sickle cell anemia: functional asplenia
Glycocalyx
Pili and fimbria
- Neisseria: recurrent infections
Flagellum
Plasmids
Ribosomes
Spores
- like seeds
Spore forming bacteria
Shapes
- vibrio: curved
Pleomorphic and obligate intracellular:
Cocci
Rods
Stains
.
- gram +: purple (Positive for Purple)
- gram -: red
- Iodine: all cells become purple
- Decolorization: only gram + retain color
- mycobacteria: acid fasting..
Giemsa
.
Ziehl-Neelsen
Aka acid fasting.
.
Silver stain
.
India Ink
- used for fungi in yeast form
.
- actinomyces: long branching, jaw abscess
